Pha-pro Integrations Direct

: P. putida KT2440 ΔfadBA + phaC1 from P. aeruginosa under Pₐₗkᵦ Feed : Waste frying oil (C18:1) Process integration :

| Component | Prokaryotic Host | Integration Strategy | |-----------|----------------|----------------------| | | Cupriavidus necator (PHB), Pseudomonas spp. (mcl-PHA) | Fuse PhaC with phasin (PhaP) for granule binding; use dual-plasmid systems | | Carbon flux | TCA cycle ↔ fatty acid β-oxidation | Knockout fadB (β-oxidation) + overexpress fadD (acyl-CoA ligase) | | Precursor supply | Acetyl-CoA → 3HB (PHB) or (R)-3HA (mcl-PHA) | Integrate phaA , phaB operon under inducible promoter (e.g., P₁₅₅₃, Pₗₐc) | pha-pro integrations

| Domain | Tool | |--------|------| | | JMP (Design of Experiments), RBS Calculator (Salis lab) | | Fermentation control | Sartorius BIOSTAT, Applikon, or Arduino-based bioreactor control with Python | | Off-gas analysis | BlueSens (CO₂/O₂ sensors), Hiden HPR-40 | | Process data integration | Python (pandas, scipy) + SQLite or OPC-UA to SCADA | | PHA analytics | GC-FID (Agilent), SEC (GPC), FTIR (Bruker Alpha II) | (mcl-PHA) | Fuse PhaC with phasin (PhaP) for

For government and public housing agencies, MRI PHA Pro is a compliance and voucher management tool. Its integrations focus on streamlining the administrative lifecycle: phaB operon under inducible promoter (e.g.

Here are some potential text-based integrations for Pha-Pro: