However, Diva users moving to spectral often struggle: there's no "compensation" to calculate. Instead, you do unmixing. The mental shift from "spillover" to "spectral signature" is real.

Veteran tip: Save your workspace BEFORE deleting anything. And then save it again.

[BD FACSDiva Workspace] │ ├── Browser Window (Hierarchical Data Storage) │ └── Folder ➔ Experiment ➔ Specimen ➔ Tubes │ ├── Cytometer Workstation (Hardware Link via Sybase SQL) │ └── CS&T Tracking ➔ Photomultiplier Voltages & Area Scaling │ └── Worksheet Canvas (Data Visualization) └── Normal Worksheets & Global Worksheets