Kawanami Spermmania | Saki

| Component | Description | |-----------|-------------| | | Drosophila melanogaster (fruit fly) – a classic model for sperm competition, plus a secondary comparative set of three beetle species ( Carabus nemoralis , Gyrinus natator , Tenebrio molitor ) to test generality. | | Experimental Design | 1. Sperm phenotyping : high‑speed video microscopy + CASA (Computer‑Assisted Sperm Analysis) to measure velocity, curvature, and longevity. 2. Genetic manipulation : CRISPR‑Cas9 knock‑ins to create lines that over‑express either fast‑type or long‑survival‑type sperm proteins. 3. Female tract simulation : microfluidic chambers replicating viscosity gradients observed in vivo. 4. Paternity assays : fluorescently labeled sperm (GFP/RFP) and next‑generation sequencing of offspring to quantify fertilization success. | | Statistical Modeling | Bayesian hierarchical models (Stan) incorporating random effects for male genotype, female genotype, and interaction terms. Model comparison via WAIC and posterior predictive checks. | | Computational Simulations | Agent‑based model (ABM) written in NetLogo, calibrated with empirical parameters, to explore long‑term dynamics of sperm type frequencies under varying mating systems. |

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If you have institutional access, the DOI link will take you directly to the PDF. Otherwise, you can request a or contact the author (email provided above) for a pre‑print. | Component | Description | |-----------|-------------| | |